Fig. 5
From: Focus on your locus with a massively parallel reporter assay
A cartoon example of a response MPRA. A In traditional MPRA, MPRA constructs introduced to the cells are not expressed because TF2 that acts on the element of interest is not translocated to the nucleus. Barcode expression of these alleles is displayed in a box plot. B In response MPRA, cells are treated with a drug X that activates downstream pathways to translocate response-dependent TF2 to the nucleus. Allele C disrupts the TF2 binding motif, and its unique barcode is not expressed, while allele A matches the TF2 motif leading to expression of its unique barcode. The barcode expression of each allele is displayed in an accompanied box plot. Therefore, this variant displays allelic regulatory activity only in response to drug X treatment