Down-regulation of the brain-specific cell-adhesion molecule contactin-3 in tuberous sclerosis complex during the early postnatal period

Table 1 Summary of sample information for comparisons between TSC and controls. Among cortical tubers used for RT-qPCR were surgery-derived (n = 30) and autopsy-derived (n = 5); front – frontal, temp – temporal, par – parietal cortex; WB – western blot samples; m – male, f – female

Summary of samples for RT-qPCR (all ages)
Sample	N	Median age	male	female	Region of brain	Mutation
Postnatal controls	27	85 months	12	15	front - 14, temp - 8, par - 1, cortex - 4	N/A	N/A
Cortical tubers	35	115 months	17	19	front - 26, temp - 9	TSC1 - 9	TSC2 - 26
Samples for WB (young TSC vs control)
Postnatal controls		Age	Gender		Region of brain	Mutation
WB CTRL1		6 weeks	m		front	–
WB CTRL2		7 weeks	f		front	–
WB CTRL3		4 months	m		front	–
WB CTRL4		7 months	f		cortex	–
WB CTRL5		1 year	f		temp	–
WB CTRL6		2 years	f		temp	–
WB CTRL7		3 years	m		front	–
Resected cortical tubers		Age	Gender		Region of brain	Mutation
WB TSC1		8 months	m		temp	TSC2
WB TSC2		1 year	m		front	TSC2
WB TSC3		2 years	m		front	TSC2
WB TSC4		2 years	m		front	TSC2
WB TSC5		2 years	f		front	TSC1
WB TSC6		3 years	f		temp	TSC2
WB TSC7		3 years	m		front	TSC2
Samples for immunohistochemistry
Postnatal controls		Age	Gender		Region of brain	Mutation
IHC CTRL1		2 years	f		front	–
IHC CTRL2		2 years	m		front	–
IHC CTRL3		10 years	m		front	–
IHC CTRL4		15 years	m		front	–
Resected cortical tubers		Age	Gender		Region of brain	Mutation
IHC TSC1		2 years	m		front	TSC2
IHC TSC2		4 years	f		front	TSC1
IHC TSC3		9 years	m		front	TSC2
IHC TSC4		13 years	f		front	TSC2
IHC TSC5		16 years	m		front	TSC2

ISSN: 1866-1955